NettetLinearized compact bacterial vector that employs a toxic minigene for high-efficiency cloning and subsequent in vitro transcription of PCR products. ... Home Resources … NettetLinearized pMiniT™ 2.0 Vector -20 1 x 0.02 ml 25 µg/ml Cloning Analysis Forward Primer -20 1 x 0.035 ml 100 µM Cloning Analysis Reverse Primer -20 1 x 0.035 ml 100 µM Amplicon Cloning Control -20 1 x 0.01 ml 15 µg/ml ...

NEB® PCR Cloning Kit NEB

NettetLeft plate serves as the control, with vector backbone only, right plate contains PCR insert. Cloning Kit Protocol Overview pMiniT 2.0 Vector Map Map shown above displays the construct formed if no insert is present. Unique restriction sites are shown in black. Additional restriction sites that can be used for subcloning are shown in red. NettetLinearized pMiniT ™ 2.0 Vector 20 µl 20 µl Amplicon Cloning Control (1 kb) 10 µl 10 µl Cloning Analysis Forward Primer 35 µl 35 µl Cloning Analysis Reverse Primer 35 µl 35 … buffett still won invest

pMiniT Sequence and Map - SnapGene

NettetProduct name Linearized pMiniT 2.0 Vector Other means of identification Product No N0312 Synonyms None Recommended use of the chemical and restrictions on use Recommended use This product is for research and development only Restrictions on use No information available Details of the supplier of the safety data sheet Emergency … NettetpMiniT (linearized) Linearized compact bacterial vector that employs a toxic minigene for high-efficiency cloning of PCR products. Sequence Author : New England Biolabs Nettet24. okt. 2016 · FAQ: What is the difference between the original pMiniT and the pMiniT 2.0 linearized vector backbone now provided in the kit? The 2.0 version features three … buffetts stocks and bonds