Supporting cells were marked by expression of GFP under the control of the p27Kip1 gene inserted into a bacterial artificial chromosome27 (BAC) containing 130 kb of DNA from the p27Kip1 locus28. The BAC was used to produce transgenic mice in which five copies of the transgene were present. See more Cochlea were removed and dissociated for FACS purification. Purity of the resulting supporting cells was assessed by re-sorting of live cells, by immunohistochemistry, … See more Purified postnatal supporting cells from wild-type or p27Kip1 mutant mice29 were grown essentially as previously described10. Purified supporting cells were plated with embryonic periotic mesenchymal cells … See more Total RNA was isolated from FACS-purified samples consisting of 10,000 cells each. PCR reactions were carried out in triplicate. Relative quantification of gene expression was … See more WebMay 7, 2024 · While the body of evidence provided in this study supports our principal conclusion that supporting cell stiffness is required for cochlear mechanics, the precise mechanism by which GAS2 mediates …
Cisplatin-induced ototoxicity in organotypic cochlear cultures …
WebFeb 14, 2024 · The most common cause of hearing loss is progressive because cochlear hair cells—the primary cells to detect sound waves—cannot regenerate if damaged or lost. People who have repeated... WebAug 21, 2024 · Cochlear hair cell loss is a leading cause of deafness in humans and other mammals. In the immature cochlea, lost hair cells are regenerated by neighboring glia-like supporting cells. However, for reasons unknown, such regenerative capacity is rapidly lost as supporting cells undergo maturation. meesho rto charges
Central role of supporting cells in cochlear homeostasis …
WebEpiphycan is specifically expressed in cochlear supporting cells and is necessary for normal hearing. Author links open overlay panel Yukiko Hanada a b, Yukiko Nakamura a, Yusuke Ishida a, Yasumitsu Takimoto a b c, Manabu Taniguchi d, Yoshiyuki Ozono b, Yoshihisa Koyama a, Tetsuo Morihana b e, Takao Imai b, Yumi Ota b, Takashi Sato b, … WebApr 29, 2024 · The mammalian cochlea loses its ability to regenerate new hair cells prior to the onset of hearing. In contrast, the adult vestibular system can produce new hair cells in response to damage, or by reprogramming of supporting cells with the hair cell transcription factor Atoh1. WebSupporting: 3, Mentioning: 12 - It is critical for hearing that the descending cochlear efferent system provide a negative feedback to hair cells to regulate hearing sensitivity and provide the protection of hearing from noise. Here, we report that the medial olivocochlear (MOC) efferent nerves, which project to outer hair cells (OHCs), also could innervate … name of addams family butler